Table 1

Dicistronic analysis of IRES activity of poly(A) and poly(G) sequences used as an intercistronic spacers

SpacerActivity in RRL assay (%)Activity in HeLa cells (%)
Poly(A)6015010
Poly(G)6011
IRESEmbedded Image100100
No RNA (mock)0.3–0.80.5–1.0
  • The H-GFP-spacer-GUS constructs were used in dicistronic assays. Relative GUS activity was expressed as in ref. 23; IRESEmbedded Image activity was taken as 100%. The mean values of three independent experiments are given.