Table 1

Inhibition of cdk-2 and lamin A/C gene expression in different human cell lines

Cells% of CDK-2-negative/GFP-positive cells % of Lamin A/C-negative/GFP-positive cells
BS/U6BS/U6/cdk-2BS/U6BS/U6/lamin A/C
HeLa0.2  ± 0.4 (n = 6)97.7  ± 1.0 (n = 6)2.0  ± 0.8 (n = 4)95.2  ± 2.1 (n = 4)
H12994.0  ± 0.5 (n = 3)86.9  ± 1.5 (n = 3)4.3  ± 1.7 (n = 3)93.5  ± 1.8 (n = 3)
U-2 OS5.2 (n = 2)92.4 (n = 2)1.2 (n = 2)93.8 (n = 2)
C-33A3.7 (n = 2)92.3 (n = 2)n.d.n.d.
  • Cells were cotransfected with CMV-GFP and either BS/U6, BS/U6/cdk-2, or BS/U6/lamin A/C and were analyzed by immunofluorescence for endogenous CDK-2 or lamin A/C expression. For each data point, 200 GFP-positive cells were scored for the presence of CDK-2 or lamin A/C signals. The values correspond to the percentage of GFP-positive cells that display a reduction of the CDK-2 or lamin A/C immunostaining near the background level and are presented as the average ± SD. n, number of transfection experiments. n.d., not determined.