Table 1

Fatty acid composition of WT and PUFA-deficient mutants

WTfat-1fat-2fat-3fat-4elo-1
Short/branch*11.611.012.912.913.011.8
Cyclopropane16.515.914.821.717.018.7
18:06.86.02.23.15.55.1
18:1n91.71.824.33.12.42.1
18:1n720.619.536.629.620.122.3
18:2n63.84.63.8 + 3.412.72.44.1
18:3n61.01.2n.d.§n.d.2.012.4
18:3n30.9n.d.n.d.11.00.40.6
18:4n3n.d.n.d.n.d.n.d.n.d.6.3
20:3n64.413.80.81.0 + 1.68.21.1
20:4n61.721.7n.d.n.d.n.d.n.d.
20:4n38.7n.d.n.d.n.d.24.83.3
20:5n319.1n.d.1.9n.d.n.d.7.8
Total PUFAs39.641.39.926.339.931.5
  • Data are mol% and represent the average of at least eight independent determinations. SEM did not exceed 7%. Alleles used are as described in the Fig. 3 legend. 

  • * Short/branched fatty acid are the total mol% of 14:0, C15:iso, C16:iso, 16:0, C17:iso, 16:1n7, and 17:0. 

  • Cyclopropane fatty acids are the sum of 9,10-methylene hexadecanoic acid and 11,12-methylene octadecanoic acid. 

  • fat-2 contains undetectable 18:2n6. Values given are for 18:2n9 and 18:2n7. 

  • § n.d., not detected (<0.3%). 

  • fat-2 contains undetectable 20:3n6. Value given is for 20:2n9. 

  • fat-3 contains undetectable 20:3n6. Values given are for 20:2n6 and 20:3n3.