Table 2

Substrate specificity of recombinant SNO in comparison to native SNO

SubstrateActivity with SNO, %
NativeRecombinant
Pyrrolizidine alkaloids
Senecionine type
 Senecionine100100
 Seneciphylline9593
 Senkirkinen.d.n.d.
Monocrotaline type
 Monocrotaline9294
 Axillarine7483
Lycopsamine type
 Heliotrine2549
Triangularine type
 Sarracine<1<10
Phalaenopsine type
 Phalaenopsinen.d.n.d.
Necine bases and other substrates
 Retronecinen.d.n.d.
 Supinidinen.d.n.d.
 Atropinen.d.n.d.
 Coffeinen.d.n.d.
l-Prolinen.d.n.d.
 Dimethylanilinen.d.n.d.