Table 1.

RNA-binding analyses of Dazl

DazlRNA *Kd, nMKrel
32–117UUGUUCUU63.6 ± 2.4
32–132UUGUUCUU38.2 ± 1.91
32–132UUGUUUUU38.0 ± 1.91.0
32–132UUCUUCUU126.4 ± 3.83.3
32–132UUAUUCUU122.9 ± 3.83.2
32–132UUGAUCUU210.2 ± 8.05.5
32–132UUGUACUU249.5 ± 7.96.5
32–132UUUUUUUU85.2 ± 4.22.2
P39AUUGUUCUU153.0 ± 6.84.0
G111AP112AUUGUUCUU170.6 ± 4.64.5
P39AG111AP112AUUGUUCUU353.4 ± 8.29.3
R115GUUGUUCUU> 2,000
32–117AAUUGUACAUAA> 2,000
32–132AAUUGUACAUAA> 2,000
  • *Bases that differ from UUGUUCUU are indicated in boldface.

  • Krel reports the affinity of Dazl32–132 for the RNA relative to that of Dazl32–132 for the 8-nt RNA UUGUUCUU.

  • Saturable binding could not be achieved at the highest protein concentration; therefore, a lower limit for the Kd is reported.