Table 2.

Dissociation constants for binding of NFR5, NFR1, WGA, and GFP to (GlcNAc)5 and Nod factor, respectively, determined by SPR and MST

SPRMST
Protein(GlcNAc)5Nod factorLinker Alexa546(GlcNAc)5 Alexa546Nod factor Alexa546
NFR510.1 ± 2.5 nM4.0 ± 1.5 nM
NFR14.9 ± 1.3 nM0.6 ± 0.25 nM
WGA25 ± 8 μM15 ± 3 μMn/an/an/a
GFPn/an/an/a
  • —, not detectable; n/a, not applicable.