Table 3.

Deep sequence analysis of 3Dpol region

WTP48KS164PA239GY268HY268WI230FF232Y
E35GE35GE35GE35GE35GE35GE35GE35G
N37GN37GN37GN37GN37GN37GN37GN37G
M145L (16%)
I165K
K170E (2.5%)
230L (1.5%), 230I (0.5%)
232F (6%)
H273RH273RH273RH273RH273RH273RH273RH273R
S299T (17%)S299T (19%)
A372V (4%)A372V (19%)
E427K
  • Passage 3 virus stocks used in these studies were used to generate libraries for deep sequencing; ∼4 million Illumina reads per virus were obtained, giving a minimum of 100,000-fold coverage of each nucleotide site. Alignments were used to identify amino acid changes representing at least between 0.5% and 1.5% of total reads (listed in white). In lighter gray, minority variants representing over 1% of the total population are shown. In dark gray, reversions at the originally altered amino acid are shown.