Table 2.

Site-directed mutagenesis on residues involved in conformational changes during catalysis

Desired variantStatus*
Active site closure for catalysis
 I230ANonviable
 I230VReversion
 I230FStable
 I230WNonviable
 F232WNonviable
 F232YStable
 F232LReversion
 F232VReversion
 F232QNonviable
 F232SNonviable
 Y234WNonviable
 Y234LNonviable
 Y234VNonviable
 Y234ANonviable
 D238NNonviable
 D238ANonviable
 D238SNonviable
 D238ENonviable
 I306WNonviable
 I306FNonviable
 L344WNonviable
 L344FNonviable
Linkage between palm and thumb
 S240WNonviable
 S240NNonviable
 S240QNonviable
 S240YNonviable
 S240FNonviable
 F246WNonviable
 F246LNonviable
 Y268WStable
 Y268HStable
 Y268ANonviable
 L269WNonviable
 L269FReversion
 M287FNonviable
 M287QNonviable
 M287HNonviable
 M287INonviable
RNA template and NTP positioning
 R174KNonviable
 R174HNonviable
 I176LNonviable
 I176VStable
 I176YNonviable
 S289WNonviable
 S289QNonviable
 S289NNonviable
 S289DNonviable
 S289CNonviable
  • *The status indicates whether the mutation was stable over three passages (dark grey), reverted to WT (lighter grey), or did not yield viable virus (lightest grey).