Table 1.

RNA melting temperatures (TM) at pH 5 and 7

RNA with predicted or known ENE*pH 7pH 5
TM,1TM,2TM,1TM,2
KSHV PAN ENE core + A32GA256 ± 176.8 ± 0.649.0 ± 0.777 ± 1
MALAT1 ENE+A WT60.9 ± 0.276.6 ± 0.666.2 ± 0.375 ± 1
MALAT1 ENE+A GC to AA45 ± 176.5 ± 0.446.7 ± 0.675.7 ± 0.3
MENβ ENE+A WT63.3 ± 0.583.9 ± 0.769.0 ± 0.986.3 ± 0.3
MENβ ENE+A GC to AA41.9 ± 0.583 ± 146.3 ± 0.485.6 ± 0.6
  • *Schematics of RNA structures are shown in Fig. 2E

  • Melting temperatures are reported in degrees Celsius as the average of three independent measurements ± SD.