Table 2.

Binding and thermodynamic parameters for UCHL1WT, UCHL1GLU7ALA, UCHL1CYS90SER, and UCHL1ILE93MET

ParametersUCHL1WTUCHL1GLU7ALAUCHL1CYS90SERUCHL1ILE93MET
kcat (s−1)0.188 ± 0.0060.017 ± 0.005ND0.084 ± 0.003
Kd (nM)*85 ± 31646 ± 28832 ± 629 ± 5
kcat/Kd (nM−1⋅s−1)2.2 × 10−32.6 × 10−5ND2.8 × 10−3
  • Values shown are the average of three different experiments ± SD. kcat, catalytic constant; Kd, dissociation constant; ND, not detectable.

  • *Kd values for all proteins were determined by isothermal titration calorimetry.