Table 1.

Structural parameters for individual proteins and complexes

Protein or ComplexRg (Å)Dmax (Å)kDa (obs)kDa (calc)χ2
CUB1CUB220.5 ± 0.1769 ± 525 ± 0.7281.7
CPIII-His35.3 ± 0.22130 ± 578 ± 0.9861.5
CPIII-Long39.5 ± 0.12152 ± 588 ± 1.2961.6
CUB1CUB2: CPIII-His40 ± 0.5130 ± 5107 ± 0.91142.7 (1.6, 2.3)
CUB1CUB2: CPIII-Long41.8 ± 0.16148 ± 5112 ± 0.91242.3 (1.7, 1.8)
  • Errors for radius of gyration Rg and kDa (obs) are standard deviations (N at least 10) based on estimates derived from Guinier plots. Errors in maximum diameter Dmax are based on multiple GNOM runs. Typical χ2 values are shown for the ab initio modeling. For individual proteins, fits were calculated using DAMMIF. For complexes, fits were calculated using MONSA, where the first figure shows the χ2 value for the complex, and the figures in parentheses refer to each separate component (CUB1CUB2 followed by CPIII-His or CPIII-Long). Obs, observed; calc, calculated.