Table 1.

Effect of metabolic inhibitors on the anisotropy of cytoplasmic GFP and membrane-localized GFP-TonB

ConstructAgentSample sizeΔRaveSDz90%*95%99%
BN1071/pGTCCCP52+0.0470.0302+11.3172PassPassPass
BN1071/pTpGCCCP23−0.0150.0431−1.6627FailFailFail
ΔexbBD/pGTCCCP30−0.0050.0177+0.052FailFailFail
BN1071/pGTNaN358+0.0600.0371+12.3404PassPassPass
BN1071/pTpGNaN340+0.0190.0674+1.7975FailFailFail
BN1071/pGTDNP53+0.0560.0512+7.9198PassPassPass
BN1071/pTpGDNP44−0.0120.0717−1.0806FailFailFail
BN1071/pGTFeEnt510.0080.0240+2.3815PassPassFail
  • BN1071 harboring pTpG or pGT, and BN1071 ΔexbBD/pGT were grown in MOPS minimal medium, suspended in PBS, and subjected to fluorescence microscopy. We measured anisotropy (R) in individual cells adhered to the coverslip before and after the addition of the noted agents to the cuvette. In each case, we conducted z-tests for paired dependent samples to determine the statistical significance of the different mean values.

  • * The observation is deemed statistically significant if |z| > 1.960.

  • The observation is deemed statistically significant if |z| > 2.241.

  • The observation is deemed statistically significant if |z| > 2.807.