Table 2.

Native-state pKa values for the acidic residues in HP36 WT and the mutants

ResidueWT HP36 native-state pKa valueK70M native-state pKa valueK65M native-state pKa valueK48M native-state pKa value
D443.04 ± 0.123.03 ± 0.073.03 ± 0.063.23 ± 0.07
E453.95 ± 0.024.13 ± 0.104.15 ± 0.094.68 ± 0.09
D463.44 ± 0.113.34 ± 0.053.33 ± 0.053.38 ± 0.05
E724.37 ± 0.034.43 ± 0.024.36 ± 0.144.41 ± 0.03
C terminus3.17 ± 0.143.13 ± 0.082.91 ± 0.102.90 ± 0.06
  • The uncertainties are the SE to the fits. Experiments were performed at 25 °C in 90% (vol/vol) H2O, 10% (vol/vol) D2O, 10 mM sodium acetate, and 150 mM sodium chloride.