Table 1

Sequence context of the asymmetric methylation found at the SUP locus within 15 cloned PCR products of bisulfite-treated DNA

Site% methylation of site
Wild typecmt3-7drm1 drm2drm1 drm2 cmt3-7No. of sites
All sites*16.25.78.70204
CpA17.14.68.7070
CpT229.513.0086
CpC4.70.71.0048
ApC8.60.64.5046
TpC18.37.18.9083
GpC13.34.75.8031
CpC22.49.114.7044
CpNpC22.58.412.7061
CpCpC40.015.630.409
  • The genotypes analyzed were either the unmutagenized clk-st line (wild-type), or clk-st containing the cmt3-7 and/or drm1 drm2 mutations. 

  • * Asymmetric methylation (All sites) are cytosines within the context CpHpH, where H = A, T, or C. In each context shown, the methylated cytosine is in bold. 

  • N = A, T, C, or G.