Table 2. mRNA levels on days 5, 7, 10, and 14 of differentiation of mMAPCs to neuroectoderm
Gene Day 0 Day 5 Day 7 Day 10 Day 14
Sox-1* 0 0.57; 1.79; 2.22 0.58; 1.15; 2.14 0.68; 0.59; 5.58 0.9; ND; 1.74
Otx-2* 0 0.11; 0.25; 3.4 0.21; 0.06; 4.2 0.12; 0.08; 2.4 ND; ND; 3.5
Otx-1* 0.01 0.33; 1.18; 7.06 0.36; 0.69; 32.1 0.26; 0.33; 24 1.67; ND; 5.31
Pax-2* 0 6.0; 8.2; 3.07 4.47; 7.06; 5.43 2.39; 1.64; 2.05 5.5; ND; 4.99
Pax-5* 0 0.11; 0.14; 0.9 0.13; 0.16; 3.75 0.05; 0.03; 1.84 0.48; ND; 1.33
En-1* 4.87 0.59; 1.45; 4.36 0.2; 0.8; 11.4 0.15; 0.32; 8 0.4; ND; 1.08
cRet 0.14 2.84; 5.46; 15.7 2.19; 5.08; 72.8 2.53; 4.47; 54.2 24, 17; ND; 36.9
Nurr-1 0 0.55; 2.4; 0.38 1.12; 1.47; 1.11 1.41; 3.59; 1.6 ND; ND; 1.68
Nestin* 0.52 27.3; 98.0; 50.4 10.2; 88.6; 70.8 5.9; 12.2; 243 6.48; ND; 14.7
GFAP 0 0.56; 0.52; 1.05 0.36; 0.52; 3.26 1.32; 5.76; 21.0 11.75; ND; 9.19
MBP 0 0.004; 0.006; 0.013 0.003; 0.008; 0.006 1.7; 4.08; 1.8 1.8; ND; 2.33
GABA 0 1.36; 1.89; 6.23 0.99; 1.74; 17.5 7.8; 19.77; 94.0 61.8; ND; 69.6
DAT 0 1.58; 4.39; 19.9 0.5; 2.83; 22.71 1.81; 5.66; 119 13.4; ND; 23.67
TH 0 0.67; 1.31; 0.36 0.5; 1.2; 2.86 1.27; 3.94; 2.54 1.7; ND; 2.29
TrH 0 0.49; 1.6; 0.46 0.51; 1.04; 4.99 1.56; 3.56; 7.14 3.92; ND; 3.81
DBH* 0 0 0 0 0
  • RNA was extracted from MAPCs, MAPCs differentiated for 5, 7, 10, and 14 days, and brain from E18 or adult mice. Contaminating DNA was eliminated by two sequential steps of DNAse treatment, mRNA was reverse-transcribed, and cDNA was amplified for 40 rounds of amplification with SYBR green PCR master mix. Controls consisted of amplifications without reverse transcription and reactions without addition of cDNA template. mRNA levels were normalized by using GAPDH as housekeeping gene and compared with levels in E18 fetal brain or adult mouse brain.

  • * Relative abundance in cultured cells compared with fetal brain.

  • Relative abundance in cultured cells compared with adult brain.