Table 4.

Competitive reverse genetics experiments

Competitive reverse genetic experimentsOrigin of reverse genetic plasmidNo. of purified viruses analyzedIncorporation rate of EN gene (%)
PB1PB2PAHANPNAMNS
C1MOMOMOMO/ENMOMOMOMO960
C2MOMOMOMOMOMO/ENMOMO9620.8
C3MOMOMOMO/HACENMOMOMOMO9644.0
C4MOMOMOMO/ENMOMOENMO9660.0
C5MOMOMOMO/ENMOMOMC5MOMO960
C6MOMOMOMO/ENMOMOMC3MOMO9656.0
  • 293T cells were transfected with plasmids for the generation of vRNAs and viral proteins of the gene segments indicated in the table. These plasmid combinations allowed competition between the HAMO and HAEN vRNAs in the presence of the MMO, MEN, MC5MO, or MC3MO vRNAs (rows C1, C4, C5, and C6, respectively), competition between the NAMO and NAEN vRNAs in the presence of the HAMO segment (row C2), or competition between the HAMO and HACEN vRNAs in the presence of the MMO vRNA (row C3). Genotypes of the plaque-purified viruses were determined by strain-specific PCR.