Table 1.

Genotypes of reassortant viruses obtained by cotransfection of EN (H5N2) and MO (H3N2) viruses

GenotypeGene originFrequency (no. viruses)
PB1PB2PAHANPNAMNS
R1MOENENENENMOENMO8
R2MOMOENENMOMOENMO5
R3ENENENENENMOENMO3
R4ENENENENENMOENEN3
R5ENMOENENENENENMO1
R6MOMOENENMOENENEN1
No of reassortant viruses possessing the respective EN segment/total no. of HAEN viruses7/21 (33%)14/21 (67%)21/21 (100%)21/21 (100%)15/21 (71%)2/21 (9%)21/21 (100%)4/21 (19%)
  • 293T cells were transfected with 16 reverse genetics plasmids corresponding to the eight gene segments of the MO and EN viruses. The cotransfection supernatants were plaque purified on MDCK cells. The genotypes of the plaque-purified viruses were determined by strain-specific PCR.