Table 2.

Heritable mutation rates using nos-Cas9 and different sgRNA plasmid constructs

SgRNA promotersgRNA scaffoldEmbryos, nG0 adultsOverall heritable mutation rate (individual), % median (range)
Total nSurvival, %Fertile, nGerm-line mutants, n (%)*
U6bShort783646.2286 (21.4)3.2 (1.1–64.6)
U6aShort681725.054 (80.0)2.1 (1.2–6.8)
nos-miniShort56916.1700
U6bLong641828.1134 (30.8)1.7 (1.5–10.0)
  • * The percentage of germ-line mutants was calculated as the proportion of fertile G0 flies that gave rise to white-eyed progeny.

  • The overall heritable mutation rate was calculated as the number of white-eyed F1s divided by the number of all F1s observed. Mutation rates for each G0 fly that generated white-eyed progeny are shown in parentheses and are calculated as the number of white-eyed F1s divided by the total F1s observed from a single G0 fly.