Table 1.

Binding constants for the fluorescein-labeled synthetic peptides corresponding to pS910, pS935, and the newly identified 14-3-3 binding region of LRRK2 containing pS1444 to three different 14-3-3 isoforms derived from FP measurements

Kd (nM) ± SD
Peptide14-3-3 gamma14-3-3 theta14-3-3 zeta
pS910661 ± 1214,100 ± 4161,336 ± 318
pS935
pS1444216 ± 44938 ± 85653 ± 93
  • Kd determinations were carried out as detailed in the Fig. S2 legend. Kd values were derived from seven independent experiments (three protein preparations), each performed in triplicate.