Table 1.

Dissociation constants of the PDZ domains of Scribble with selected peptides as determined using synthetic fluorescein-labeled peptides

KD (μM)
ProteinPeptidePDZ1PDZ2PDZ3
Human−6−5−4−3−2−10
 B7Z2Y1RFLETKL2.1 ± 0.229 ± 55.8 ± 0.6
 ARHG7AWDETNL2.3 ± 0.317 ± 23.5 ± 0.2
 NXPE2VQRHTWLNA5 ± 17 ± 2
 PKP4GSPDSWVNB37 ± 722 ± 5
 DNM1LIRETHLWNBNA1.1 ± 0.4
 MK12VSKETPLNANA5.0 ± 0.5
 GCYA2FLRETSLNANA10 ± 2
 CTNB1AWFDTDLNANA8.5 ± 2
 METASFWETSNANANB
Viral
 TAX HTL1LHFHETEVNA7 ± 22.5 ± 0.7
  • NA, not available as the dissociation constants were not determined; NB, no binding under conditions used. No binding was observed with the scrambled NATWLED peptide used as negative control.