Table 1.

Map of gating pore mutations

PositionGASTCVLIMPFYWDENQHKR
V236+++++*+++++++++
I237+++++++*++++++
S240++*+++++++++++
I241*H+
F244+++++++++*+••+++
C286++++*+++++++++
I287+++••••••+*+++++••••H+
F290••••••••••••••••*••••••••
A319*+++++++++++++
I320+++++++*++++
  • The table shows new functional mutants analyzed in this study (+), mutants that were partially (•) or fully characterized (••) in previous studies, nontested mutants (blanks), nonfunctional mutants (), and mutants previously shown to permeate protons at hyperpolarized voltages (H+). In each row, the asterisk (*) indicates the native residue.