Table 1.

Kd values of Ply and Ply mutants with glycans determined by SPR

GlycanPlyPly306PlyL460DPlyW433FPlySPlyQ374APlyY376A
LeX3.17 (±1.70)2.89 (±1.56)n.i.8.25 (±0.444)*2.62 (±2.15)2.24 (±0.204)3.41 (±0.471)
sLeX1.88 (±0.423)35.0 (±56.3)n.i.13.9 (±2.09)*4.30 (±0.543)13.7 (±2.03)*19.4 (±2.69)*
  • Mean Kd values of at least three runs ± 1 SD are reported as 10−5 M. Lactose and LeB were included in SPR analysis. No interactions were observed with these glycans and any Ply sample. n.i., no interaction (Kd observed was greater than 1 × 10−3 M and/or the χ2 value was greater than 50% of the maximum response).

  • * Significant difference in mean Kd value compared with WT Ply with the same glycan (P < 0.05).