Table 1.

Specific activities (nmol product/μg protein/min) of labeled and unlabeled proteins

PocketMutationUnlabeled activityLabeled activity
Background (M182T)532 ± 5
1L286C361 ± 2997 ± 6
2A232C545 ± 75255 ± 21
2A249C193 ± 13150 ± 18
3S203C520 ± 13345 ± 17
SurfaceA150C235 ± 25249 ± 28
BuriedL190C213 ± 22
BuriedI260C267 ± 38
  • The reduction in activity on labeling demonstrates that there is communication between the proposed allosteric sites and the active site. The pockets are (1) the known allosteric site, (2) the first predicted site, and (3) the second predicted site.