Table 2.

Model scenarios based on sex ratio skews in combined clotrimazole exposure, temperature, and breeding treatments

No.Emergent inbreeding depressionExposure scenarioAge 0+ survivorshipOverall proportion of females per treatment, %Mean proportion of females per family per tank compartment per treatment, % (SD)
1aInbred, zero clotrimazole, 28 °CLow4241 (28)
1b(Inbred control)
2aOutbred, zero clotrimazole, 28 °CHigh5758 (30)
2b(Outbred control)
3aInbred, zero clotrimazole, 33 °CLow2728 (33)
3b
4aOutbred, zero clotrimazole, 33 °CHigh5252 (34)
4b
5aInbred, high clotrimazole, 28 °CLow1918 (18)
5b
6aOutbred, high clotrimazole, 28 °CHigh4040 (30)
6b
7aInbred, low clotrimazole, 33 °CLow1818 (22)
7b
8aOutbred, low clotrimazole, 33 °CHigh5151 (26)
8b
9aInbred, high clotrimazole, 33 °CLow33 (8)
9b
10aOutbred, high clotrimazole, 33 °CHigh1817 (26)
10b
  • Exposure scenarios represent treatment combinations in the exposure study. Clotrimazole geometric mean measured concentrations: control (0 μg/L), low (1.7 μg/L), high (8 μg/L). First-year (age 0+) survivorship: low = 4 ± 3% for inbreds, high = 9 ± 3% for outbred zebrafish (SI Appendix, Table S7). Proportions of females per family (tank compartment) per treatment were used for statistical analysis (arcsine-square root transformed data) and PVA.