Table 1.

Experimental treatments

Clotrimazole nominal (and measured) exposure concentrations, μg/LInbred zebrafish families (FIT = n + 0.25)Outbred zebrafish families (FIT = n)
28 °C33 °C28 °C33 °C
0 (0)
2 (1.7)
10 (8.0)
  • FIT = n (unknown quantity) for outbreds and FIT = n + 0.25 for inbreds following one generation of full-sibling mating.

  • ✔Replication for each treatment was n = 18 inbred families and n = 20 outbred families. Each family replicate per treatment was represented by n = 4 fish.

  • ✗This “low dose × low temperature” treatment was not included in our analyses because we knew, from our previous work (40, 83), that the low-dose clotrimazole (2 μg/L) alone would not affect population-relevant end points (survival, growth, gonadal development or fecundity), and we sought to maximize replication for the other treatment regimes.