Table 3.

Transgene insertion following suppression of NHEJ

TargetHR armsdsRNANo. injectedNo. G0 (%)No. G0 poolsNo. G1 screenedEGFP+ (#)Rate
kmo0.6/0.5 kb1,010109 (11%)6>11,0000<1.8%
kmo2.2/1.7 kb1,030152 (15%)8>12,0000<1.3%
kmo2.2/1.7 kbegfp1,130192 (17%)9∼33,0000<1.0%
kmo2.2/1.7 kbku70 #11,090166 (15%)9∼33,000P1 (1), P3 (36)2.4%
kmo2.2/1.7 kbku70 #21,035118 (11%)5∼16,000P2 (8)1.7%
dcr21/1.7 kbegfp1,138237 (21%)10∼46,0000<0.8%
dcr21/1.7 kbku70 #21,050144 (14%)7∼23,000P1 (7), P2 (2)2.7%
Total-/egfp3,298581 (18%)27∼91,0000<0.3%
ku703,175428 (13%)21∼72,00052.3%
  • Minimum rate of gene knockin based on 50% fertility.