Table 2.

CRISPR mutagenesis is highly efficient in Ae. aegypti

TargetsgRNANo. injectedTotal G0Fertile G0Mutant G1
kmo519*830170 (20.5%)84 (49.4%)27/85 (32.1%)
lig4#71,110176 (15.8%)ND8/23 (34.8%)
ku70#3613172 (28%)77 (44.8%)10/42 (23.8%)
r2d2#6565121 (21.4%)51 (42.1%)20/33 (60.6%)
loqs#3548124 (22.6)62 (50%)27/30 (90.0%)
  • ND, not determined.

  • Number of G0 individuals that produced mutant progeny/total.