Table 1.

Tn-seq assays

Mutant poolTn-seq runColonies pooledMapped readsUnique insertion sitesEssential coding genes
LB-11139,1002,303,02594114468
2139,10013,436,77788202485
3139,1008,997,56563546561
LB-21106,40014,904,26981677429
2106,40018,640,54281550495
LB-31172,80024,322,071109951467
Minimal-11240,65028,214,377193993421
Minimal-21312,00013,427,239205614406
Minimal-31285,8005,160,085252948450
Sputum-1176,4005,661,97079723446
Sputum-21144,0007,630,737181477378
Sputum-3146,80014,522,50048635564
Sputum-4196,6002,992,34091843469
0.1X LB1180,8008,736,912121357423
Human Serum1155,400754,804127933488
BHI1216,0004,439,772174631483
Total2,172,7501,043,276*
  • The Tn-seq results for 16 experiments corresponding to 13 independent mutant pools are summarized. The essential coding genes for each run were combined to identify consensus essentials for each media type.

  • * Corresponds to one insertion every 6.0 bp.