Table 1.

Spontaneous can1 mutations in msh6Δ pol3-R696W yeast strains

MutationNo. of detectable mutationsMutation rate (×10−7)*Percentage of total
Base substitutions
 GC to AT1391,10073
 GC to TA2419012
 GC to CG18.00.5
 AT to GC141108
 AT to CG4322
 AT to TA7564
−1 frameshift18.00.5
 Total1901,500100
  • The data are based on DNA sequence analysis of 189 independent CanR mutants of msh6Δ pol3-R696W strains containing pPOL3. Fourteen mutants contained two different point mutations in the CAN1 gene. For these mutants, only mutations previously reported to produce a detectable CanR phenotype are shown; the others were identified as likely hitchhiker mutations and were excluded from the mutation rate calculation. The location of mutations in the CAN1 sequence is shown in Fig. S1.

  • * The rate for each type of mutation was calculated as follows: MRi = (Mi/MT) × MR, where Mi is the number of mutations of the particular type, MT is the total number of mutations, and MR is the rate of CanR mutation in msh6Δ pol3-R696W strains containing pPOL3 as determined by fluctuation analysis (1,500 × 10−7; Table S1).