Table S2.

Plasmids used in this study

Plasmid nameRelevant genotypeSource
pHT4LEU2 pCUP1::GBP-RFPPresent study
pHT10LEU2 pCUP1::MTW1-GBP-RFPPresent study
pHT296LEU2 pCUP1::MTW1Present study
pHT353LEU2 pCUP1::GBP-RFP-CDC14Present study
pHT354LEU2 pCUP1::CDC14Present study
pHT355LEU2 pCUP1::GBP-RFP-cdc14-C283SPresent study
pHT356LEU2 pCUP1::GBP-RFP-cdc14-C283APresent study
pHT411LEU2 pCUP1::CDC14-GBP-RFPPresent study
pHT412LEU2 pCUP1::cdc14-C283S-GBP-RFPPresent study
pHT99NAT pCUP1 (empty)Present study
pHT369NAT pCUP1::DSN1Present study
pHT379NAT pCUP1::dsn1-S264DPresent study
pHT380NAT pCUP1::dsn1-S240D, S250DPresent study
pHT381NAT pCUP1::dsn1-S69D, S170D, S240D, S250D, S264D (5D)Present study
pHT382NAT pCUP1::dsn1-S69D, S170D, S264D (3D)Present study
pHT274LEU2 pCUP1::NUF2-GBP-RFPPresent study
pHT210LEU2 pCUP1::NUF2Present study
pHT314LEU2 pCUP1::MIF2-GBP-RFPPresent study
pHT313LEU2 pCUP1::MIF2Present study
pHT312LEU2 pCUP1::CTF19-GBP-RFPPresent study
pHT311LEU2 pCUP1::CTF19Present study
pHT340LEU2 pCUP1::KRE28-GBP-RFPPresent study
pHT339LEU2 pCUP1::KRE28Present study
pHT336LEU2 pCUP1::SKP1-GBP-RFPPresent study
pHT335LEU2 pCUP1::SKP1Present study
pHT344LEU2 pCUP1::CBF1-GBP-RFPPresent study
pHT310LEU2 pCUP1::CBF1Present study
pHT319LEU2 pCUP1::CNN1-GBP-RFPPresent study
pHT318LEU2 pCUP1::CNN1Present study
pHT342LEU2 pCUP1::CHL4-GBP-RFPPresent study
pHT341LEU2 pCUP1::CHL4Present study
pHT338LEU2 pCUP1::CTF3-GBP-RFPPresent study
pHT337LEU2 pCUP1::CTF3Present study
pHT239LEU2 pCUP1::HTA2-GBP-RFPPresent study
pHT238LEU2 pCUP1::HTA2Present study
pHT11LEU2 pCUP1::SPC42-GBP-RFPPresent study
pHT297LEU2 pCUP1::SPC42Present study
pHT230LEU2 pCUP1::ASE1-GBP-RFPPresent study
pHT229LEU2 pCUP1::ASE1Present study
pHT431LEU2 pCUP1::CDC14-GBP(no RFP)Present study
pHT432LEU2 pCUP1::cdc14-C283A-GBP(no RFP)Present study
  • All plasmids are based upon pWJ1512 (32), which contains CEN6 and ARS209.