Table S1.

Yeast strains used in this study

Strain nameGenetic backgroundRelevant genotypeSource
PT95-19CW303*MATa ADE2 TRP1 FOB1-CFPPresent study
PT19-1CW303MATa HTA1-CFP::NATPresent study
W8164-2BW303MATα CEN1-16::Gal-Kl-URA3(32)
PT73-7AW303MATa CSE4-CFP::HIS3Present study
PT129-1AW303MATα ADE2 LYS2 SPC110-CFP::KAN RAD5Present study
GFP strainsBY4741MATa his3∆1 leu2∆0 met15∆0 ura3∆0 XXX-GFP::HIS3(20)
T317BY4741MATa CDC14-GFP::HIS3 ubr2∆::NATPresent study
T306BY4741MATa NDC10-GFP::HIS3 ubr2∆::NATPresent study
T307BY4741MATa BIR1-GFP::HIS3 ubr2∆::NATPresent study
T310BY4741MATa NNF1-GFP::HIS3 ubr2∆::NATPresent study
T311BY4741MATa DAD2-GFP::HIS3 ubr2∆::NATPresent study
T309BY4741MATa NSL1-GFP::HIS3 ubr2∆::NATPresent study
T312BY4741MATa DAD2-GFP::HIS3 ubr2∆::NATPresent study
T308BY4741MATa NUF2-GFP::HIS3 ubr2∆::NATPresent study
PT142-1CW303MATα ADE2 TRP1 leu2-3,112 his3-11,15 ura3-1 lys2∆ RAD5 ASK1-CFPPresent study
PT95-35AW303MATα ADE2 trp1-1 leu2-3,112 his3-11,15 FOB1-CFPPresent study
PT39-30AW303MATα ADE2 TRP1 leu2-3,112 his3-11,15 ura3-1 lys2∆ RAD5 MTW1-CFPPresent study
PT149W303/BY4741MATα/MATa FOB1-CFP CDC5-GFP::HIS3Present study
PT150W303/BY4741MATα/MATa FOB1-CFP TOM70-GFP::HIS3Present study
PT151W303/BY4741MATα/MATa FOB1-CFP YPR174C-GFP::HIS3Present study
PT152W303/BY4741MATα/MATa FOB1-CFP NUP1-GFP::HIS3Present study
PT153W303/BY4741MATα/MATa FOB1-CFP CDC14-GFP::HIS3Present study
PT154W303/BY4741MATα/MATa MTW1-CFP CDC14-GFP::HIS3Present study
PT155W303/BY4741MATα/MATa ASK1-CFP CDC14-GFP::HIS3Present study
PT156W303/BY4741MATα/MATa ASK1-CFP CDC5-GFP::HIS3Present study
PT157W303/BY4741MATα/MATa ASK1-CFP TOM70-GFP::HIS3Present study
PT158W303/BY4741MATα/MATa ASK1-CFP YPR174C-GFP::HIS3Present study
PT159W303/BY4741MATα/MATa ASK1-CFP NUP1-GFP::HIS3Present study
T376BY4741MATa NNF1-GFP::HIS3 DSN1-3HA::HYGPresent study
T81BY4741MATa CSE4-GFP::HIS3 mad3∆::KANPresent study
T82BY4741MATa CDC20-GFP::HIS3 mad3∆::KANPresent study
T83BY4741MATa NDC10-GFP::HIS3 mad3∆::KANPresent study
T84BY4741MATa IPL1-GFP::HIS3 mad3∆::KANPresent study
T85BY4741MATa BIR1-GFP::HIS3 mad3∆::KANPresent study
T414BY4741MATa NUF2-GFP::HIS3 mad3∆::KANPresent study
T415BY4741MATa NNF1-GFP::HIS3 mad3∆::KANPresent study
T416BY4741MATa MTW1-GFP::HIS3 mad3∆::KANPresent study
T417BY4741MATa CDC14-GFP::HIS3 mad3∆::KANPresent study
T418BY4741MATa KRE28-GFP::HIS3 mad3∆::KANPresent study
T419BY4741MATa NSL1-GFP::HIS3 mad3∆::KANPresent study
T420BY4741MATa CEP3-GFP::HIS3 mad3∆::KANPresent study
T421BY4741MATa DAD3-GFP::HIS3 mad3∆::KANPresent study
T422BY4741MATa BIK1-GFP::HIS3 mad3∆::KANPresent study
T423BY4741MATa BUB3-GFP::HIS3 mad3∆::KANPresent study
T424BY4741MATa MIF2-GFP::HIS3 mad3∆::KANPresent study
T425BY4741MATa CBF1-GFP::HIS3 mad3∆::KANPresent study
T426BY4741MATa SLI15-GFP::HIS3 mad3∆::KANPresent study
T427BY4741MATa AME1-GFP::HIS3 mad3∆::KANPresent study
T428BY4741MATa MCM21-GFP::HIS3 mad3∆::KANPresent study
T429BY4741MATa CTF19-GFP::HIS3 mad3∆::KANPresent study
T430BY4741MATa STU2-GFP::HIS3 mad3∆::KANPresent study
T431BY4741MATa DSN1-GFP::HIS3 mad3∆::KANPresent study
T432BY4741MATa mtw1∆::KAN with plasmid pHT10 [MTW1-GBP LEU2]Present study
T449W303MATα CEN1-16::Gal-Kl-URA3 CDC14-GBP-RFP::KANPresent study
PT209-7AW303MATa ura3::3xURA3-tetOx112 TetR-mRFP(iYGL119W) MTW1-YFPPresent study
  • * W303 strains are ADE2+ RAD5+ derivatives of W303 (can1-100 his3-11, 15 leu2-3,112 ura3-1), unless otherwise indicated (56, 57).

  • GFP strains are all based upon BY4741 [his3∆1 leu2∆0 met15∆0 ura3∆0 (20)].