Table 4.

Comparison of free energy changes with respect to angle from stalling experiment with theoretical predictions

Reaction stepProperties*ExperimentTheory
λΔG00θfθidlnKdθdlnkfdθκdlnkfdθκ
ATP binding566.080°0.910.45200.4216
GTP binding555.480°0.840.47200.4715
  • * Free energies are given in kcal/mol. Properties are estimated from experiment and are independent of columns 4–6.

  • dlnkf/dθ and dlnK/dθ are in units of 1/10° (14). κ is in units of pN nm (4).