Table S2.

Thermodynamic parameters for ion competition in KcsA channels

ChannelpHLigand (X+)Kd(X+), μMKd(Na+), mMn
WT-ΔC4Rb+51 ± 214 ± 41
OM1 (H25R-E118A-E120A)-ΔC8K+120 ± 2250 ± 101
OM2 (H25Q-E118+)-ΔC8K+31 ± 613 ± 21.4 ± 0.03
E71C-ΔC8K+83 ± 513 ± 12.6 ± 0.1
M96V-ΔC8K+350 ± 400.48 ± 0.161.6 ± 0.1
R64A-ΔC8K+54 ± 917 ± 21.4 ± 0.1
WT-FL8K+67 ± 49 ± 11