Table 1.

Comparison of various DNA polymerase activities with different template-primers and divalent cations

Source of DNA polymeraseActivity (3H cpm incorporated × 10–3) with:
Poly(A)⋅(dT)12–18Poly(dA)⋅(dT) 12–18Poly(C)⋅(dG) 12–18
Mg2+Mn2+Mg2+Mn2+Mg2+Mn2+
HTLVCR104762102210
SSV25371.51260
NC37-α1412930.50.5
NC37-γ1444114133250.52
  • Assays were performed with a 10l aliquot of the indicated DNA polymerase sample with either 10 mM Mg2+ or 0.25 mM Mn2+ as the divalent cation. The Mg2+ and Mn2+ concentrations are optimized for HTLVCR RT with poly(A)⋅(dT)12–18 and are not necessarily the optimal divalent cation concentrations for the other DNA polymerases tested.