Table S1.

Effects of triple-MAGUK knockdown measured by electrophysiology and EM tomography

MeasurementsControlKnockdownPercentage of reduction, %
AMPAR EPSC amplitude, pA89.7 ± 19.9 (n = 10)22.9 ± 7.2 (n = 10)73
NMDAR EPSC amplitude, pA24.8 ± 5.2 (n = 10)9.3 ± 2.3 (n = 10)61
AMPAR mEPSC amplitude, pA12.9 ± 0.6 (n = 8)10.8 ± 0.7 (n = 8)16
AMPAR mEPSC frequency, Hz4.0 ± 1.1 (n = 8)0.8 ± 0.1 (n = 8)81
PSD length, nm331 ± 93 (n = 143)158 ± 103 (n = 252)52
Synapse contact length, nm665 ± 203 (n = 77)598 ± 198 (n = 114)10 (NS)
PSD area, μm2 (STEM-tomo)10.10 ± 0.06 (n = 70)0.032 ± 0.042 (n = 172)68
PSD diameter, nm (STEM-tomo)352 ± 95 (n = 53)237 ± 94 (n = 107)33
Synapse contact area, μm2 (STEM)0.25 ± 0.1 (n = 51)0.21 ± 0.15 (n = 109)16 (NS)
Density vertical filaments, no./0.1 μm2 (TEM-tomo)368 ± 21 (n = 5)187 ± 85 (n = 19)49
Density AMPAR structures, no./0.1 μm2 (TEM-tomo)135 ± 66 (n = 5)27 ± 32 (n = 19)80
Cluster diameter of NMDAR-type structures, nm (TEM-tomo)220 ± 10 (n = 3)116 ± 17 (n = 12)47
Nearest neighbor distance
 NMDAR structures, nm (TEM-tomo)30 ± 2 (n = 63)31 ± 5 (n = 197)−3 (NS)
 AMPAR structures, nm (TEM-tomo)27 ± 3 (n = 52)49 ± 61 (n = 72)−81
Vertical filaments, no. per PSD (based on density; TEM-tomo)383* ± 22*60* ± 27*∼84
AMPAR no. per PSD (based on density; TEM-tomo)105* ± 52*21* ± 25*∼80
AMPAR no. per partial PSD (TEM-tomo)227∼68
NMDAR no. (based on NMDAR cluster size; TEM-tomo)∼75
NMDAR no. per partial PSD23 ± 5 (n = 12)12 ± 3 (n = 12)∼48
(TEM-tomo)
  • All measurements are expressed as mean± SD unless otherwise stated. STEM-tomo, STEM tomography; TEM-tomo, TEM tomography.

  • * Estimates based on density of vertical filaments or AMPAR-type structures using average PSD area/diameter measured by STEM tomography excluding the area of PSD occupied by NMDAR-type structures assuming no other structural changes at the postsynaptic membrane.

  • Direct measurements of no. of AMPAR- or NMDAR-type of structures from tomograms of ∼100-nm-thick PSD stubs.