Table S4.

SPR analysis of glycan–glycan interactions using free host glycans and bacterial surface glycans isolated from NTHi

GlycanNTHi 2019 WT LPS, µMNTHi 2019 WT Ode-PS, µMNTHi 2019 ΔsiaP-LPS, µMNTHi 2019 ΔsiaP Ode-PS, µM
Lewis A8.86 ± 0.980.09 ± 0.031.09 ± 0.070.835 ± 0.31
Lewis XNCDINCDINCDINCDI
A trisaccharide77.9 ± 5.32.97 ± 1.1NCDINCDI
B trisaccharide42.1 ± 3.02.64 ± 0.26NCDINCDI
H (O) disaccharide80.3 ± 7.73.95 ± 0.433.71 ± 0.362.93 ± 0.93
Chondrotin sulfate (15 kDa)NCDI1.19 ± 0.39NCDINCDI
Hyaluronin (107 kDa)NCDI1.36 ± 0.26NCDINCDI
SialyLewis XNCDINCDINCDINCDI
  • NCDI, no concentration-dependent interaction observed within the range of the instruments detection. Ode-PS indicates the polysaccharide obtained from O-deacylation of LPS from NTHi strains.