Table S1.

Mycobacterial strains used in this study

Strain nameGenotypeHow constructedSource
Parental strains
  H37RvWTN/ATrudeau Institute
  mc26230H37Rv ΔRD1 ΔpanCDSpTr and unmarking(24, 25)
H37Rv background
 Hygromycin-marked strains
  mc27788Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27789ΔesxG (Rv0287)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27790ΔesxH (Rv0288)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27791ΔesxGesxH (Rv0287Rv0288)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27792Δpe5ppe4 (Rv0285–Rv0286)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27808ΔmbtB (Rv2383c)::res-sacB-hygR-resSpTr of H37RvThis work
  mc27809Δmbt-1 (Rv2377cRv2386c)::res-sacB-hygR-resSpTr of H37RvThis work
 Hygromycin-marked, complemented strains
  mc27827Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-res, attBL5::pYUB1335Transformation (pYUB1335)This work
  mc27828Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-res, attBL5::pYUB2076Transformation (pYUB2076)This work
  mc27829Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-res, attBL5::pJP148Transformation (pJP148)This work
  mc27830ΔesxG (Rv0287)::res-sacB-hygR-res, attBL5::pJP148Transformation (pJP148)This work
  mc27831Δesx-3 (Rv0282–Rv0292)::res-sacB-hygR-res, attBL5::pMV361Transformation (pMV361)This work
 Unmarked strains (sacB-hygR cassette removed)
  mc27844Δesx-3 (Rv0282Rv0292)::resPhage delivery of γδ-resolvaseThis work
  mc27845ΔesxG (Rv0287)::resPhage delivery of γδ-resolvaseThis work
  mc27846ΔesxH (Rv0288)::resPhage delivery of γδ-resolvaseThis work
  mc27847ΔesxGesxH (Rv0287-Rv0288)::resPhage delivery of γδ-resolvaseThis work
  mc27848Δpe5ppe4 (Rv0285-Rv0286)::resPhage delivery of γδ-resolvaseThis work
  mc27850ΔmbtB (Rv2383c)::resPhage delivery of γδ-resolvaseThis work
 Double-deletion strains (second deletion introduced into unmarked strain)
  mc27851ΔesxGesxH (Rv0287Rv0288)::res; Δmbt-1 (Rv2377c–Rv2386c)::res-sacB-hygR-resSpTr of mc27847
  mc27852Δesx-3 (Rv0282Rv0292)::resmbt-1 (Rv2377cRv2386c)::res-sacB-hygR-resSpTr of mc27844
 Unmarked complemented strains
  mc27856Δesx-3 (Rv0282Rv0292)::res, attBL5::pYUB1335Phage delivery of γδ-resolvaseThis work
  mc27857Δesx-3 (Rv0282Rv0292)::res, attBL5::pYUB2076Phage delivery of γδ-resolvaseThis work
  mc27866ΔesxG (Rv0287)::res, attBL5::pYUB1944Transformation (pYUB1944)This work
  mc27867ΔesxH (Rv0288)::res, attBL5::pYUB1944Transformation (pYUB1944)This work
  mc27868Δpe5ppe4 (Rv0285-Rv0286)::res, attBL5::pYUB1945Transformation (pYUB1945)This work
  mc27874ΔmbtB (Rv2383c)::res, attBL5::pYUB1940Transformation (pYUB1940)This work
mc26230 background
 Hygromycin-marked strains
  mc27818Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-resSptr of mc26230This work
  mc27819ΔesxH (Rv0288)::res-sacB-hygR-resSptr of mc26230This work
  mc27820ΔmbtB (Rv2383c)::res-sacB-hygR-resSptr of mc26230This work
 Hygromycin-marked complemented strains
  mc27838Δesx-3 (Rv0282Rv0292)::res-sacB-hygR-res, attBL5::pYUB1335Transformation (pYUB1335)This work
 Unmarked strains (sacB-hygR cassette removed)
  mc27860Δesx-3 (Rv0282Rv0292)::resPhage delivery of γδ-resolvaseThis work
  mc27861ΔesxH (Rv0288)::resPhage delivery of γδ-resolvaseThis work
  mc27862ΔmbtB (Rv2383c)::resPhage delivery of γδ-resolvaseThis work
 Unmarked complemented strain
  mc27863Δesx-3 (Rv0282Rv0292)::res, attBL5::pYUB1335Phage delivery of γδ-resolvaseThis work
  • The table lists strain names and relevant genotypes for the mycobacterial strains used in this study; the methods of construction are also indicated as well as the source where appropriate. SpTr, specialized transduction.