Table S2.

Plasmids and cosmids used in this study

PlasmidRelevant genotype/descriptionSource
pYUB412attPL5 λcos int hyg bla ColE1; mycobacterial cosmid, replicates as an episomal vector in Escherichia coli, and integrates into the attBL5 sites of various mycobacterial species.S. Bardarov and W. R. Jacobs Jr.
pYUB1052attPL5 λcos int aph bla ColE1; derivative of pYUB412, with hyg cassette replaced with aph gene conferring kanamycin resistance (kanR)(78)
pYUB1335pYUB1052::(Rv0278c [portion] to Rv0303 [portion]); cosmid vector containing Mtb esx-3 regionThis work
pYUB2076pYUB1052::(MSMEG_0609 [portion] to MSMEG_0637 [portion]); cosmid vector containing M. smegmatis esx-3 region(14)
pYUB1940pYUB412::(Rv2374c [portion]-Rv2387 [portion]); cosmid vector containing Mtb mbt-1 regionThis work
pMV261aph oriM Phsp60 ColE1; episomal mycobacterial shuttle vector, contains hsp60 promoter(79)
pMV361aph int attPL5 Phsp60 ColE1; integrating mycobacterial shuttle vector, contains hsp60 promoter(79)
pMV306aph int attPL5 ColE1; integrating mycobacterial shuttle vector(79)*
pJP148pMV361::esxGMtb-esxHMtb ; esxG (Rv0287)-esxH (Rv0288) of Mtb H37Rv cloned between EcoRI and HindIII sites of pMV361This work
pYUB1942pMV261::esxGMtb-esxHMtb ; esxG (Rv0287)-esxH (Rv0288) of Mtb H37Rv cloned between MscI and HindIII sites of pMV261This work
pYUB1943pMV261::pe5Mtb-ppe4Mtb ; pe5 (Rv0285)-ppe4 (Rv0286) of Mtb H37Rv cloned between MscI and HindIII sites of pMV261This work
pYUB1944pMV306:: Phsp60-esxGMtb-esxHMtb ; XbaI/HindIII fragment of pYUB1942 subcloned into XbaI/HindIII-digested pMV306 vector backbone.This work
pYUB1945pMV306:: Phsp60-pe5Mtb-ppe4Mtb ; XbaI/HindIII fragment of pYUB1943 subcloned into XbaI/HindIII-digested pMV306 vector backbone.This work
  • The table lists plasmids and cosmids used in this study, including the relevant genotypes and the source where appropriate.

  • * Derivative of pMV361 lacking Phsp60.