Table S2.

Sample size, mixed-effects model parameters, and estimated effects for data presented in Fig. 2, ADH activity at ZH-86Fb from F2s using high throughput method and no-intercept model

Sample sizeRandom effectsFixed effectsPlanned comparisons
Day repsConstruct-zygosityN (lines)Vials per lineTotal samplesSDUnequal variance multiplierEstimateSEt valuedfP
1Dup_hom3316Line0.009Dup_hom7.86Dup_hom1.2640.042Dup_het vs. Single_hom7.9940.0013
Dup_het3327Vial0.020Dup_het4.02Dup_het0.7430.018
Single_hom3322Residual0.021Single_hom4.65Single_hom0.5150.022
Single_het3317Single_het1Single_het0.2810.009
Uninserted (pooled)6Uninserted0.4Uninserted0.0760.009
  • Model: lme(relative_ADH_activity ∼ -1 + construct_zygosity, random = list(one1 = pdIdent(∼line-1), one2 = pdIdent(∼vial-1)), weights = varIdent(form=∼1|construct_zygosity)). For details, see the legend of Table S1.