Table S6.

Plasmids used in this study

PlasmidDescriptionMarker(s)Source
pNPTS138For two-step recombination-mediated gene excision and replacementnptI (KanR), sacB (SucS)(67)
pMCS-1For chromosomal integration at any genomic locus of interestaadA (SpecR)(70)
pMCS-2For chromosomal integration at any genomic locus of interestnptI(70)
pXCHYC-1For generating chromsomal transcriptional fusions to the xylX promoter (PxylX)aadA(70)
pVCHYC-2For generating chromsomal transcriptional fusions to the vanA promoter (PvanA)nptI(70)
pXCHYC-2For generating chromsomal transcriptional fusions to the xylX promoter (PxylX)nptI(70)
pRXMCS-2Low-copy replicating vector with PxylX promoter for xylose-inducible overexpressionnptI(70)
pRXMCS-6Low-copy replicating vector with PxylX promoter for xylose-inducible overexpressioncat (ChlorR)(70)
pRVMCS-2Low-copy replicating vector with PvanA promoter for vanillate-inducible overexpressionnptI(70)
pBXMCS-2High-copy replicating vector with PxylX promoter for xylose-inducible overexpressionnptI(70)
pBAD33Replicating vector with ParaBAD promoter for arabinose-inducible expressioncat(72)
pUT18CFor generating IPTG-inducible fusions of CyaAT18 to the N terminus of a gene of interestbla (AmpR)Euromedex
pKT25For generating IPTG-inducible fusions of CyaAT25 to the N terminus of a gene of interestnptIEuromedex
pUT18C-ZIPPositive control plasmid for two-hybrid assay (gives Lac+ phenotype w/ pKT25-ZIP)blaEuromedex
pKT25-ZIPPositive control plasmid for two-hybrid assay (gives Lac+ phenotype w/ pUT18C-ZIP)nptIEuromedex
pXCHYC-2::gapRFor single-step integration of gapR at the xylX locusnptIThis study
pVCHYC-2::gapRFor single-step integration of gapR at the vanA locusnptIThis study
pNPTS138::∆gapR::ΩFor deletion of gapR and replacement with the Ω cassettenptI aadAThis study
pXCHYC-1::sspBFor single-step integration of E. coli sspB at the xylX locusaadAThis study
pMCS-2::'gapR-EcssrAADASFor single-step tagging of chromosomal gapR with the E. coli ssrAADAS tagnptIThis study
pMCS-2::'ctrA-EcssrAADASFor single-step tagging of chromosomal ctrA with the E. coli ssrAADAS tagnptIThis study
pMCS-2::'gapR-M2-EcssrAADASFor single-step tagging of chromosomal gapR with FLAG and E. coli ssrAADAS tagsnptIThis study
pMCS-2::'gapR-M2For single-step tagging of chromosomal gapR with a C-terminal FLAG tagnptIThis study
pMCS-2::PgapR-M2-gapR'For single-step tagging of chromosomal gapR with an N-terminal FLAG tagnptIThis study
pMCS-2::'ctrA-M2For single-step tagging of chromosomal ctrA with a FLAG tagnptIThis study
pXCHYC-2::gapR-mCherryFor single-step integration of gapR-mCherry at the xylX locusnptIThis study
pXCHYC-2::mCherry-gapRFor single-step integration of mCherry-gapR at the xylX locusnptIThis study
pXCHYC-2::mCherry-gapRRhodoFor single-step integration of mCherry-gapRRhodo at the xylX locusnptIThis study
pXCHYC-2::eGFP-hnsFor single-step integration of eGFP-hns at the xylX locusnptIThis study
pRMCS-2::PgapR-gapRFor low-copy expression of gapR from its native promoternptIThis study
pBMCS-2::PgapR-gapRFor high-copy expression of gapR from its native promoternptIThis study
pRXMCS-6::gapRFor low-copy expression of gapR from the PxylX promotercatThis study
pBXMCS-2::gapRFor high-copy expression of gapR from the PxylX promoternptIThis study
pRVMCS-2::gapRFor low-copy expression of gapR from the PvanA promoternptIThis study
pRVMCS-2::gapRAgroFor low-copy expression of gapRAgro from the PvanA promoternptIThis study
pRVMCS-2::gapRRhodoFor low-copy expression of gapRRhodo from the PvanA promoternptIThis study
pRVMCS-2::gapRSinoFor low-copy expression of gapRSino from the PvanA promoternptIThis study
pRVMCS-2::gapRΦCr30For low-copy expression of gapRΦCr30 from the PvanA promoternptIThis study
pRVMCS-2::hnsFor low-copy expression of hns from the PvanA promoternptIThis study
pBAD33::hnsFor arabinose-inducible expression of hnscatThis study
pBAD33::gapRFor arabinose-inducible expression of gapRcatThis study
pBAD33::mCherry-gapRFor arabinose-inducible expression of mCherry-gapRcatThis study
pBAD33::eGFP-hnsFor arabinose-inducible expression of eGFP-hnscatThis study
pBAD33::mCherry-gapR_eGFP-hnsFor arabinose-inducible expression of eGFP-hns and mCherry-gapR (synthetic operon)catThis study
pBAD33::mCherryFor arabinose-inducible expression of mCherrycatThis study
pKT25::gapRFor IPTG-inducible expression of GapR fused to the C terminus of CyaAT25nptIThis study
pUT18C::gapRFor IPTG-inducible expression of GapR fused to the C terminus of CyaAT18blaThis study
pKT25::gapRΦCr30For IPTG-inducible expression of GapRΦCr30 fused to the C terminus of CyaAT25nptIThis study