Table S1.

Sugar analyses of cell walls from Mtb H37Rv, ΔcpsA, and the complemented strain

StrainRhaAraManGalGlcGlcNAcGalNAcMur
H37Rv1.0032.352.7315.8813.744.801.503.28
ΔcpsA1.0037.449.6620.5019.736.791.607.44
ΔcpsA::cpsA1.0032.034.2016.8714.455.331.672.48
  • The amount of rhamnose (Rha), an arabinogalactan marker, was compared with the amount of GlcNAc and N-acetyl muramic acid (Mur) (peptidoglycan markers) in the indicated strains. The amounts of each monosaccharide in the samples are expressed relative to Rha, which is set to be constant. Consistent with earlier analysis of Mtb and M. marinum strains deficient in the expression of cpsA, the data reflect a slight loss of arabinogalactan attachment to peptidoglycan in the mutant strain that is restored toward WT levels in the complemented strain. Ara, arabinose; Gal, galactose; Glc, glucose; Man, mannose.