Table S2.

Protein kinase sites with decreased phosphorylation and with documented effects on enzyme activity

Gene symbolAnnotationUniProtModification siteCentralized sequencePhosphorylation PKA dKO/Ctrl, mean ± SDKinase classRegion from AbDesignerEffect of phosphorylation from Kinexus
Clk1Dual-specificity protein kinase CLK1P22518S340HHSTLVS*TRHYRA0.546 ± 0.036CMGCActivation loopDecreases
Epha2Ephrin type-A receptor 2Q03145Y589LKPLKTY*VDPHTY0.428 ± 0.158TyrBetween transmembrane span and kinase domainIncreases
Epha2Ephrin type-A receptor 2Q03145Y595YVDPHTY*EDPNQA0.428 ± 0.158TyrBetween transmembrane span and kinase domainIncreases
Map2k3Dual-specificity mitogen-activated protein kinase kinase 3O09110S218SGYLVDS*VAKTMD0.534 ± 0.090STEActivation loopIncreases
Map3k5Apoptosis signal regulating kinase (ASK1)O35099S973EYLRSIS*LPVPVL0.506 ± 0.282STEDownstream from kinase domainDecreases
Map3k5Apoptosis signal regulating kinase (ASK1)O35099S1036ENFEDHS*APPSPE0.520 ± 0.110STEDownstream from kinase domainDecreases
MtorSerine/threonine-protein kinase mTORQ9JLN9S2481VPESIHS*FIGDGL0.176 ± 0.037AtypicalJust downstream from kinase domainIncreases
MtorSerine/threonine-protein kinase mTORQ9JLN9S2448SRTRTDS*YSAGQS0.583 ± 0.178AtypicalJust downstream from kinase domainIncreases
PrkcdProtein kinase C deltaP28867Y332LDNNGTY*GKIWEG0.528 ± 0.244AGCJust upstream from kinase domainInduces Src binding
Sik2Salt-inducible kinase 2Q8CFH6S358GRQRRPS*TIAEQT0.043 ± 0.008CAMKJust downstream from UBA domainIncreases
SrcNonreceptor protein tyrosine kinase SrcP05480S17ASQRRRS*LEPSEN0.515 ± 0.076TyrUpstream from SH3 domainDecreases
Srpk2Serine/arginine-rich protein-specific kinase 2O54781S50EEEILGS*DDEEQE0.570 ± 0.522CMGCJust upstream from kinase domainIncreases
Wnk1Serine/threonine-protein kinase WNK1P83741S382RASFAKS*VIGTPE0.401 ± 0.055OtherActivation loopIncreases
  • Bold indicates basophilic target sites and possible direct PKA targets.