Table S1.

Analysis of IFN protein from HIEs

IFN sample analyzed6 hpi8 hpi12 hpi20 hpi24 hpi48 hpi
IFN-λ1
 Supernatant
 Concentrated supernatant
 Supernatant + anti-IL28RA
 Cells + supernatant
IFN-λ2/3
 Supernatant
 Supernatant + anti-IL28RA
 Cells + supernatant
IFN-β1
 Supernatant*
  • MOI > 10 FFU per cell for each experiment. −, below the limit of detection. “Concentrated supernatant” indicates supernatant from n = 5–6 samples concentrated into a single sample. “Supernatant + anti-IL28RA” indicates supernatant from HIEs incubated with anti-IL28RA antibody (25 μg/mL) during infection to block the type III IFN receptor. “Cells + supernatant” indicates sonicated homogenate of HIEs and surrounding media. IFN-λ1 ELISA sensitivity is 8 pg/mL. IFN-λ2/3 ELISA sensitivity is 15.6 pg/mL. and IFN-β1 ELISA sensitivity is 50 pg/mL.

  • * Limit of detection is 2 pg/mL.