Table 1.

FWHM of the PSF measured in cryoconditions (140°C) and at room temperature by using the prototyped cryoimmersion objective (63×/1.15) and comparison with an air objective (63×/0.75)

Observation conditionsFWHMxy (nm)FWHMz (nm)Peak intensity (a.u.)
Cryoimmersion objective W2010 @ 23 °C295 ± 6902 ± 178,444 ± 484
Cryoimmersion objective HFE 7200 @ −140 °C353 ± 161,554 ± 583,449 ± 362
Air objective 0.75 NA @ −140 °C385 ± 141,743 ± 108610 ± 165