Table 1.

DXR binding affinity to NorM-Vc mutants under distinct conditions

ConstructMutant locationConditionKD ± SD,* μMN
pH 7.51.66 (0.26)6
CyslessN/ApH 7.5, 10 mM NaCl3.01 (0.22)6
pH 4.022.21 (2.83)5
A45C/L269C (P)N/ApH 7.52.52 (0.23)6
P/D36NNTD, TM1pH 7.52.64 (0.08)2
P/N174DNTD, TM5pH 7.51.66 (0.09)3
P/N178DNTD, TM5pH 7.52.34 (0.19)3
P/T200KNTD, TM6pH 7.53.92 (0.43)3
P/T200NNTD, TM6pH 7.52.29 (0.09)2
P/E255QCTD, TM7pH 7.514.80 (1.22)3
P/D371NCTD, TM10pH 7.514.07 (1.69)3
P/E255Q/D371NCTD, TM7/TM10pH 7.517.08 (2.02)3
  • * For n ≥ 3, average ± SD; for n = 2, average ± spread.

  • Total number of experiments; where n > 3, replicates include multiple protein preparations.

  • Experiments performed with spin labeled protein.