Table S2.

Panicoid rDNA types in Hordeum and Pseudoroegneria

Accession no.Amplicon 2: Total panicoid ITS rDNA amplified with a Panicoideae-specific primerGenus-specific ITS rDNA types captured in cloned amplicon 2 (no. of clones sequenced)Amplicon 3: PCR screening for individual ITS types (total no. of clones obtained for each type)
PaspalumPanicum 1Panicum 2EuclastaSetariaArundinella
Hordeum comosum JB412+− + − − − − (15)+ (3)+ (1)+ (1 Direct)+ (1)+ (1)
Hordeum comosum JB292A+− + − − − − (16)+ (4)+ (3)+ (1 Direct)+ (2)+ (1 Direct)
Hordeum patagonicum pat. BCC2031++ − − + − − (15)+ (6)+ (3)+ (3)+ (4)
Hordeum patagonicum pat. BCC2064+Not sequenced+ (3)+ (4)+ (1)+ (1)
Hordeum patagonicum set. BCC2032++ − + + − − (16)+ (2)+ (2)+ (3)+ (2)
Hordeum patagonicum set. BCC2038++ − − − + − (10)+ (1)+ (1)+ (1 Direct)+ (6)+ (14)
Hordeum patagonicum mus. BCC2033+− + − − − − (10)+ (1)+ (3)+ (1 Direct)
Hordeum patagonicum mag. BCC2065++ − − + − − (16)+ (5)+ (1 Direct)+ (4)
Hordeum pubiflorum BCC2028++ − − + − − (21)+ (14)+ (2)+ (1)+ (2)
Hordeum pubiflorum BCC2068+Not sequenced+ (3)+ (2)
Hordeum cordobense BCC2067+− + − − − − (10)+ (1)+ (10)+ (1)
Hordeum cordobense BCC2039+Not sequenced+ (3)+ (2)+ (3)
Hordeum muticum BCC2042+− + − − − − (10)+ (7)+ (3)
Hordeum muticum BCC2014+Not sequenced+ (1 Direct)+ (3)
Hordeum flexuosum BCC2023++ − − − − − (16)+ (1)+ (1)
Hordeum flexuosum JB208A+Not sequenced+ (1)
Hordeum chilense CN27413++ − − − − − (14)+ (1)
Hordeum chilense GRA1000+Not sequenced+ (1)+ (4)+ (4)
Hordeum erectifolium BCC2026++ − − − + − (22)+ (1)+ (15)
Hordeum stenostachys BCC2041+− − − − + − (14)+ (1 Direct)+ (6)
Hordeum stenostachys BCC2021+− + − + + + (16)+ (4)+ (2)+ (4)+ (4)+ (1)
Hordeum euclaston BCC2022++ − − − − − (10)+ (5)
Hordeum euclastonBCC2029+Not sequenced+ (2)+ (4)
Hordeum intercedensBCC2044++ − − − − − (13)+ (4)
Hordeum intercedens BCC2059++ − − − − − (9)+ (3)
Hordeum pusillum BCC2043++ − − − − − (9)+ (3)
Hordeum pusillum BCC2049Not sequenced
Hordeum californicum BCC2057++ − − + − − (9)+ (1)+ (3)
Hordeum californicum BCC2058Not sequenced
Hordeum bogdanii BCC2063+− + − − − − (9)+ (4)
Hordeum bogdanii BCC2070+Not sequenced+ (4)
Hordeum roshevitzii BCC2015+− + − − − − (10)+ (2)
Hordeum roshevitzii BCC2069+− + − − − − (10)+ (2)
Hordeum brevisubulatum PI401374Not sequenced
Hordeum brevisubulatum BCC2016+Not sequenced+ (1)+ (7)
Hordeum marinum BCC2001Not sequenced
Hordeum marinum BCC2006Not sequenced
Hordeum mar. gussoneanum BCC2005Not sequenced
Hordeum mar. gussoneanum BCC2012Not sequenced
Hordeum murinum glaucum BCC2002Not sequenced
Hordeum murinum glaucum BCC2017Not sequenced
Hordeum vulgare F2097Not sequenced
Hordeum vulgare HOR3094Not sequenced
Hordeum bulbosum BCC2061Not sequenced
Hordeum bulbosum (4x) GRA970Not sequenced
 Total no. of clones (and direct sequences)73 (+1)60 (+1)12 (+5)3848 (+1)5
Pseudoroegneria gracillima USDA440000Not sequenced
Pseudoroegneria libanoticaUSDA228389Not sequenced
Pseudoroegneria libanoticaUSDA401274Not sequenced
Ps. spicata USDA632464+Not sequenced+ (1 Direct)
Ps. spicata USDA563869+− − − − + − (42)+ (42)
Pseudoroegneria stipifolia USDA325181Not sequenced
Ps. strigosa USDA499637+Not sequenced+ (1 Direct)
 Total no. of clones (and direct sequences)000042 (+2)0
  • Initially, total (= native + nonnative) ITS rDNA was amplified in all Triticeae accessions (amplicon 1) (details in Materials and Methods and Fig. S1). Next, all accessions were screened with a Panicoideae-specific primer, amplifying total panicoid ITS types (amplicon 2); + and − characters denote successful and unsuccessful amplification, respectively. Cloning amplicon 2 and sequencing of clones (only in selected accessions; column 3) resulted in capture and identification of individual panicoid ITS types (+ and − denote the presence or absence, respectively, of individual ITS types corresponding to Paspalum, Panicum 1, Panicum 2, Euclasta, Setaria, and Arundinella, with the number of sequenced clones in parentheses). Next, all accessions were screened with the designed specific primers for the presence of individual panicoid ITS types (amplicon 3): presence, +; absence, −. The total number of clones obtained for that particular ITS type (including clones of amplicon 2) is given in parentheses. Direct indicates direct sequence of amplicon 3 for that accession. Additional details are in Materials and Methods and Fig. S1.