Table S1.

Kinetic data of lesion bypass by RNA pol II: Incorporation

LesionIncorporation
NTPkpol, min−1Kd, µMkpol/Kd, min−1⋅µM−1
O2-EtdTATP0.34 ± 0.037,100 ± 800(4.7 ± 0.7) × 10−5
UTP0.0095 ± 0.00104,700 ± 800(2.0 ± 0.4) × 10−6
GTP0.24 ± 0.063,900 ± 1,500(6.1 ± 2.7) × 10−5
CTP0.0087 ± 0.00056,000 ± 500(1.5 ± 0.2) × 10−6
N3-EtdTATP0.11 ± 0.013,800 ± 600(2.9 ± 0.5) × 10−5
UTP0.096 ± 0.0022,900 ± 100(3.4 ± 0.2) × 10−5
GTP0.017 ± 0.0058,300 ± 3,500(2.0 ± 1.1) × 10−6
CTP0.018 ± 0.0017,100 ± 600(2.6 ± 0.3) × 10−6
O4-EtdTATP0.12 ± 0.014,500 ± 400(2.7 ± 0.3) × 10−5
UTP0.017 ± 0.0022,600 ± 400(2.0 ± 0.4) × 10−6
GTP3.1 ± 1.27,100 ± 4,000(4.3 ± 3.0) × 10−4
CTP(4.9 ± 0.4) × 10−42,600 ± 400(1.9 ± 0.3) × 10−7