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genome engineering

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Improved bacterial recombineering by parallelized protein discovery
Timothy M. Wannier, Akos Nyerges, Helene M. Kuchwara, Márton Czikkely, Dávid Balogh, Gabriel T. Filsinger, Nathaniel C. Borders, Christopher J. Gregg, Marc J. Lajoie, Xavier Rios, Csaba Pál, and George M. Church

PNAS first published May 28, 2020. https://doi.org/10.1073/pnas.2001588117
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Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules
Yuhong Cao, Enbo Ma, Stefano Cestellos-Blanco, Bei Zhang, Ruoyi Qiu, Yude Su, Jennifer A. Doudna, and Peidong Yang

PNAS April 16, 2019 116 (16) 7899-7904; first published March 28, 2019. https://doi.org/10.1073/pnas.1818553116
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Reply to Nathamgari et al.: Nanopore electroporation for intracellular delivery of biological macromolecules
- Oct 29, 2019
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Codon usage of highly expressed genes affects proteome-wide translation efficiency
Idan Frumkin, Marc J. Lajoie, Christopher J. Gregg, Gil Hornung, George M. Church, and Yitzhak Pilpel

PNAS May 22, 2018 115 (21) E4940-E4949; first published May 7, 2018. https://doi.org/10.1073/pnas.1719375115
Open Access
Deletion of DXZ4 on the human inactive X chromosome alters higher-order genome architecture
Emily M. Darrow, Miriam H. Huntley, Olga Dudchenko, Elena K. Stamenova, Neva C. Durand, Zhuo Sun, Su-Chen Huang, Adrian L. Sanborn, Ido Machol, Muhammad Shamim, Andrew P. Seberg, Eric S. Lander, Brian P. Chadwick, and Erez Lieberman Aiden

PNAS August 2, 2016 113 (31) E4504-E4512; first published July 18, 2016. https://doi.org/10.1073/pnas.1609643113
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A scalable strategy for high-throughput GFP tagging of endogenous human proteins
Manuel D. Leonetti, Sayaka Sekine, Daichi Kamiyama, Jonathan S. Weissman, and Bo Huang

PNAS June 21, 2016 113 (25) E3501-E3508; first published June 6, 2016. https://doi.org/10.1073/pnas.1606731113
Open Access
A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species
Ákos Nyerges, Bálint Csörgő, István Nagy, Balázs Bálint, Péter Bihari, Viktória Lázár, Gábor Apjok, Kinga Umenhoffer, Balázs Bogos, György Pósfai, and Csaba Pál

PNAS March 1, 2016 113 (9) 2502-2507; first published February 16, 2016. https://doi.org/10.1073/pnas.1520040113
Open Access
Generation of knock-in primary human T cells using Cas9 ribonucleoproteins
Kathrin Schumann, Steven Lin, Eric Boyer, Dimitre R. Simeonov, Meena Subramaniam, Rachel E. Gate, Genevieve E. Haliburton, Chun J. Ye, Jeffrey A. Bluestone, Jennifer A. Doudna, and Alexander Marson

PNAS August 18, 2015 112 (33) 10437-10442; first published July 27, 2015. https://doi.org/10.1073/pnas.1512503112
Open Access
Rational design of a split-Cas9 enzyme complex
Addison V. Wright, Samuel H. Sternberg, David W. Taylor, Brett T. Staahl, Jorge A. Bardales, Jack E. Kornfeld, and Jennifer A. Doudna

PNAS March 10, 2015 112 (10) 2984-2989; first published February 23, 2015. https://doi.org/10.1073/pnas.1501698112
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Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes
- From the Cover
Mark D. Szczelkun, Maria S. Tikhomirova, Tomas Sinkunas, Giedrius Gasiunas, Tautvydas Karvelis, Patrizia Pschera, Virginijus Siksnys, and Ralf Seidel

PNAS July 8, 2014 111 (27) 9798-9803; first published May 27, 2014. https://doi.org/10.1073/pnas.1402597111
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R-loop formation mechanism of Cas9 and Cascade
- Jul 02, 2014
Open Access
Precise and in situ genetic humanization of 6 Mb of mouse immunoglobulin genes
Lynn E. Macdonald, Margaret Karow, Sean Stevens, Wojtek Auerbach, William T. Poueymirou, Jason Yasenchak, David Frendewey, David M. Valenzuela, Cosmas C. Giallourakis, Frederick W. Alt, George D. Yancopoulos, and Andrew J. Murphy

PNAS April 8, 2014 111 (14) 5147-5152; first published March 25, 2014. https://doi.org/10.1073/pnas.1323896111
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Antibodies from megabase Ig-gene humanized mice
- Mar 25, 2014

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