Manuscript Format

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PNAS Author Center

Manuscript Order

Many authors find it useful to organize their manuscript sections as follows: title page, abstract, significance statement, introduction, results, discussion, materials and methods, acknowledgments, references, and figure legends. If authors present information clearly and concisely, other variations to this format are allowed. Number all manuscript pages starting with the title page as page 1.

Title Page

Information entered in the submission form will be used for publication purposes (e.g., author contact information and affiliations). Please include the following information on the title page:

• Classification: Select a major (Physical, Social, or Biological Sciences) and a minor category from the following. Dual classifications are permitted between major categories and in exceptional cases, subject to Editorial Board approval, within a major category.
   
• PHYSICAL SCIENCES: Applied Mathematics; Applied Physical Sciences; Astronomy; Biophysics and Computational Biology; Chemistry; Computer Sciences; Earth, Atmospheric, and Planetary Sciences; Engineering; Environmental Sciences; Mathematics; Physics; Statistics; and Sustainability Science.
   
• SOCIAL SCIENCES: Anthropology; Economic Sciences; Environmental Sciences; Political Sciences; Psychological and Cognitive Sciences; Social Sciences; and Sustainability Science.
   
• BIOLOGICAL SCIENCES: Agricultural Sciences; Anthropology; Applied Biological Sciences; Biochemistry; Biophysics and Computational Biology; Cell Biology; Developmental Biology; Ecology; Environmental Sciences; Evolution; Genetics; Immunology and Inflammation; Medical Sciences; Microbiology; Neuroscience; Pharmacology; Physiology; Plant Biology; Population Biology; Psychological and Cognitive Sciences; Sustainability Science; and Systems Biology.
   
• Title: Titles should be no more than 3 typeset lines (generally 135 characters including spaces) and should be comprehensible to a broad scientific audience. The organism studied should be included.
   
• Author Affiliation: Include department, institution, and complete address, with the ZIP/postal code, for each author. Use superscripts to match authors with institutions. Authors are strongly encouraged to supply their ORCID identifier.
   
• Corresponding Author: The name, complete address, phone number, and email address of the author to whom correspondence and proofs should be sent. Email addresses will appear in the article footnotes.
   
• Keywords: Keywords are listed below the article abstract. At least 3 keywords are required at submission.

Abstract

Provide an abstract of no more than 250 words on page 2 of the manuscript. Abstracts should explain to the general reader the major contributions of the article. References in the abstract must be cited in full within the abstract itself and cited in the text.

Significance Statement

Authors must submit a 120-word-maximum statement about the significance of their research paper written at a level understandable to an undergraduate-educated scientist outside their field of specialty. The primary goal of the Significance Statement is to explain the relevance of the work in broad context to a broad readership. Significance statements are not required for Brief Reports.

Text

Describe procedures in sufficient detail so that the work can be repeated. Methods must be presented after results and discussion. Follow the spelling and usage given in Webster's Third New International Dictionary or the Random House Dictionary of the English Language. Avoid laboratory jargon. Correct chemical names should be given, and strains of organisms should be specified. Trade names should be identified by an initial capital letter with the remainder of the name lowercase. Names of suppliers of uncommon reagents or instruments should be provided. Use Système International units and symbols whenever possible. Statements of novelty and priority are not permitted in the text.

• Language-Editing Services: Prior to submission, authors who believe their manuscripts would benefit from professional editing are encouraged to use a language-editing service (see list here). PNAS does not take responsibility for or endorse these services, and their use has no bearing on acceptance of a manuscript for publication.
   
• Use of URLs in Text: As a publisher, PNAS must be able to archive the data essential to a published article. Where such archiving is not possible, deposition of data in public databases, such as GenBank, ArrayExpress, Protein Data Bank, Unidata, and others outlined in the Information for Authors, is acceptable. Only links to websites that are permanent public repositories, such as self-perpetuating online resources funded by government, academia, and industry, are permitted. Links to an author's personal web page are not acceptable.
   
• Gene Nomenclature: Prior to submission, authors should consult Genecards, MGI Nomenclature page, HUGO Gene Nomenclature Committee, or equivalent resources to ensure standardized nomenclature is used for species-specific gene and protein names. For proposed gene names, please submit the gene symbols to the appropriate nomenclature committee as these must be deposited and approved before publication of the article.
   
• Abbreviations: Abbreviations should be accessible to a broad scientific audience; abbreviations that are specific to a particular field should be defined at first mention in the text.
   
• Nomenclature and Style: Use international standards on nomenclature. PNAS uses Scientific Style and Format: The CSE Manual for Authors, Editors, and Publishers (8th edition, 2014) as the primary style guide.

Footnotes

PNAS distinguishes author affiliations and footnotes from in-text footnotes by assigning a different set of footnote symbols to each type. Superscript lowercase letters separated by commas (no spaces) are used for author affiliations. Superscript numerals separated by commas (no spaces) are used for author footnotes. In-text footnotes should be preceded by a footnote symbol, used in the order *, †, ‡, §, ¶, #, ||,**, ††, ‡‡, §§, ¶¶, ##.

Acknowledgments

List acknowledgments and funding sources; all abbreviations should be spelled out. PNAS collaborates with FundRef to use a standard taxonomy of funder names. Dedications are rarely allowed.

References

References must be in PNAS style. For references to in-press articles, please confirm with the cited journal that the article is in press and include a DOI number and online publication date. Unpublished abstracts of papers presented at meetings or references to "data not shown" are not permitted. References should be cited in numerical order as they appear in text, and all references cited in the main text should be included in the main manuscript file. Include a separate citation list for references cited in the SI. Because tables and figures will be inserted in the text where first cited, references in these sections should be numbered accordingly. Include the full title for each cited article. All authors may be named in the citation for initial submissions. For revisions or final submissions, if there are more than 5 authors, list the first author’s name followed by et al. Provide volume numbers for journal articles as applicable; provide DOI numbers if volume numbers are not available. Provide inclusive page ranges for journal articles and book chapters. Provide date of access for online sources.

Journal articles are cited as follows:

10. Neuhaus J-M, Sticher L, Meins F, Jr, Boller T (1991) A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc Natl Acad Sci USA 88:10362–10366.

Cite research datasets in the references and list the authors, title, publisher (repository name), identifier (DOI in URL format), and date of deposition as follows:

12. van Sebille E, Doblin M (2016) Data from "Drift in ocean currents impacts intergenerational microbial exposure to temperature." Figshare. https://dx.doi.org/10.6084/m9.figshare.3178534.v2 (deposited 15 April 2016).

Use MEDLINE/PubMed abbreviations of journal titles or use the full journal title for any journals not indexed in MEDLINE.

Articles or chapters in books are cited as follows:

14. Hill AVS (1991) HLA associations with malaria in Africa: Some implications for MHC evolution. Molecular Evolution of the Major Histocompatibility Complex, eds Klein J, Klein D (Springer, Heidelberg), pp 403–420.

Preprints are cited as follows with a DOI or preprint ID number, "Preprint," and the date of posting:

15. Luetkens H, et al. (2008) Electronic phase diagram of the LaO1–xFxFeAs superconductor. arXiv:0806.3533v1. Preprint, posted June 21, 2008.

Figure Legends

Provide these separately from figures, after the references in the manuscript. For figures with multiple panels, the first sentence of the legend should be a brief overview of the entire figure; each panel must be explicitly referenced and described at least once in the figure legend. Graphs should include clearly labeled error bars described in the figure legend. Authors must state whether a number that follows the ± sign is a standard error (SEM) or a standard deviation (SD). The P value, magnification, or scale bar information should be provided when applicable. The number of independent data points (N) represented in a graph must be indicated in the legend. Numerical axes on graphs should go to 0, except for log axes.

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