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Edited by Lora V. Hooper, The University of Texas Southwestern, Dallas, TX, and approved August 1, 2017 (received for review April 11, 2017)
Significance
Premature birth (PTB) is a major global public health burden. Previous studies have suggested an association between altered vaginal microbiota composition and PTB, although findings across studies have been inconsistent. To address these inconsistencies, improve upon our previous signature, and better understand the vaginal microbiota’s role in PTB, we conducted a case-control study in two cohorts of pregnant women: one predominantly Caucasian at low risk of PTB, the second predominantly African American at high risk. With the results, we were able to replicate our signature in the first cohort and refine our signature of PTB for both cohorts. Our findings elucidate the ecology of the vaginal microbiota and advance our ability to predict and understand the causes of PTB.
Abstract
Preterm birth (PTB) is the leading cause of neonatal morbidity and mortality. Previous studies have suggested that the maternal vaginal microbiota contributes to the pathophysiology of PTB, but conflicting results in recent years have raised doubts. We conducted a study of PTB compared with term birth in two cohorts of pregnant women: one predominantly Caucasian (n = 39) at low risk for PTB, the second predominantly African American and at high-risk (n = 96). We profiled the taxonomic composition of 2,179 vaginal swabs collected prospectively and weekly during gestation using 16S rRNA gene sequencing. Previously proposed associations between PTB and lower Lactobacillus and higher Gardnerella abundances replicated in the low-risk cohort, but not in the high-risk cohort. High-resolution bioinformatics enabled taxonomic assignment to the species and subspecies levels, revealing that Lactobacillus crispatus was associated with low risk of PTB in both cohorts, while Lactobacillus iners was not, and that a subspecies clade of Gardnerella vaginalis explained the genus association with PTB. Patterns of cooccurrence between L. crispatus and Gardnerella were highly exclusive, while Gardnerella and L. iners often coexisted at high frequencies. We argue that the vaginal microbiota is better represented by the quantitative frequencies of these key taxa than by classifying communities into five community state types. Our findings extend and corroborate the association between the vaginal microbiota and PTB, demonstrate the benefits of high-resolution statistical bioinformatics in clinical microbiome studies, and suggest that previous conflicting results may reflect the different risk profile of women of black race.
Footnotes
↵1B.J.C. and D.B.D. contributed equally to this work.
- ↵2To whom correspondence should be addressed. Email: relman{at}stanford.edu.
Author contributions: B.J.C., D.B.D., S.P.H., and D.A.R. designed research; B.J.C., D.B.D., D.S.A.G., C.L.S., E.K.C., P.J., J.R.B., R.J.W., M.L.D., G.M.S., D.K.S., S.P.H., and D.A.R. performed research; B.J.C., D.B.D., P.J., S.P.H., and D.A.R. contributed new reagents/analytic tools; B.J.C., D.B.D., D.S.A.G., C.L.S., E.K.C., P.J., R.J.W., S.P.H., and D.A.R. analyzed data; and B.J.C., D.B.D., D.S.A.G., C.L.S., E.K.C., J.R.B., R.J.W., M.L.D., G.M.S., D.K.S., S.P.H., and D.A.R. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
Data deposition: The sequences reported in this paper have been deposited in the NCBI Sequence Read Archive, https://www.ncbi.nlm.nih.gov/sra (accession no. SRP115697).
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1705899114/-/DCSupplemental.
Freely available online through the PNAS open access option.
http://www.pnas.org/site/misc/userlicense.xhtmlVaginal microbial signature of preterm birth
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